Y. V. Hurmach, M. P. Rudyk, V. M. Svyatetska, O. V. Skachkova, L. M. Skivka
Taras Shevchenko National University of Kyiv, Ukraine, National Cancer Institute, Kyiv, Ukraine
FUNCTIONAL PROFILE OF PHAGOCYTES FROM DIFFERENT LOCATIONS IN RAT WITH C6 GLIOMA
Glioma is one of the most common types of malignant brain tumor characterized by bad prognosis. The development of malignant glioma is accompanied by the microglial activation, the influx of circulating phagocytes and the massive infiltration of systemic macrophage-like cells as a result of a disrupted blood-brain barrier. It results in dramatic alterations of the metabolism of this complex microglia. Functional characteristics of these cells can reflect the pathological changes in the tumor microenvironment. Mucous membranes of the nose are an integral part of the lymphoid tissue associated with the mucous membranes (Mucosa Associated Lymphoid Tissue, MALT), whose structural components are closely interconnected. Resident phagocytes in mucosa associated lymphoid tissue are peritoneal macrophages. However, the data concerning metabolic state of such phagocyte cells in glioma-bearing animals are controversial and sparse. The research aims to study the functional profile of brain phagocytes and peritoneal macrophages in rats affected with C6 glioma. Phagocyte functional profile was characherized by arginase and NO-synthase activity (colorimetric assays) as well as ROS generation, phagocytosis and CD206 expression (flow cytometry). The research findings show that in vivo C6 glioma growth is associated with alterations in functional profile of both microglia and resident phagocytes in mucosa associated lymphoid tissue (MALT) – peritoneal macrophages. Peritoneal macrophages in rats with C6 glioma is characterized by alternative functional profile. Microglia has its distinctive properties as compared with M1 and M2 phagocytes. It may be caused of complexity of glioma-associated mononuclear phagocyte population.
Keywords: glioma, microglia, macrophages, polarization, functional profile