S. V. Serga, S. V. Demidov, I. A. Kozeretska
ESC «The Institute of Biology and Medicine» Taras Shevchenko National University of Kyiv, Ukraine
ENDOSYMBIOTIC BACTERIA WOLBACHIA AND OVARIOLE NUMBER IN DROSOPHILA MELANOGASTER FEMALES
Wolbachia are endosymbiotic alpha-proteobacteria, predominantly transmitted transovarially and infect a large number of invertebrates. The success of infection with these bacteria in natural populations of host species can be explained by the influence of Wolbachia on reproduction or fitness of infected individuals. Wolbachia infect Drosophila melanogaster populations worldwide, although the univocal effect of bacteria on the fitness or reproduction of the fruit fly has not been identified. In this work, we first estimated the effect of Wolbachia on such an important quantitative indicator of reproduction success as the ovariole number in the ovaries, which can affect the number of offspring, and therefore the spread of bacteria in the host population. In this study were involved two isofemale lines of D. melanogaster, which are infected with various Wolbachia genotypes, namely wMel and wMelCS. It is known that these genotypes have different effects on the fecundity of Drosophila. To obtain genetically identical infected and uninfected lines, we applied antibiotic treatment. The ovariole number was compared in lines maintained on a standard medium and those that were grown on the medium supplemented with an antibiotic tetracycline. We found that Wolbachia infection of the wMel and wMelCS genotypes causes an approximately 2-fold increase in the ovariole number in the isofemale lines from the Uman population.
Keywords: Wolbachia, Drosophila melanogaster, ovariole number, Uman