A. V. Rutska, I. Y. Krynytska
I. Horbachevsky Ternopil State Medical University, Ukraine
ENERGY METABOLISM IN RATS AFFECTED BY TOBACCO SMOKE AND LONG-TERM ADMINISTRATION OF MONOSODIUM GLUTAMATE: SEX AND AGE ASPECTS
Aim of research. To examine the processes of energy supply in rats exposed to tobacco smoke against the background of the use of monosodium glutamate in the sex and age aspects. Object and research methods. Experiments were performed on 96 white mature and immature rats of both sexes, which were kept on a standard vivarium diet. Each group of animals was divided into four subgroups: I – control (n = 8); ІІ – rats with modeled passive tobacco smoking (n = 8); ІІІ – rats, which were injected with monosodium glutamate (n = 8); IV – rats with modeled passive tobacco smoking combined with the monosodium glutamate injection (n = 8). Research results and their interpretation. It was established that passive smoking against the use of monosodium glutamate in mature male rats is accompanied by a marked inhibition of processes of energy supplying oxidation, as indicated by decrease of succinate hydrogenase activity in the mitochondria of leukocytes by 47.1% (p <0.001) relative to the control group, which is 27.9% (p<0.001) below this indicator, with the isolated effect of tobacco smoke and a decrease in cytochrome oxidase activity by 27.5% (p<0.001) relative to the control group, which did not significantly differ from this indicator provided isolated exposure to tobacco smoke. Conclusions. Taking into consideration the aspect of sex, the metabolic processes as influenced by passive smoking and monosodium glutamate are more pronounced in females, and with the age-old comparison of changes in the activity of these enzymes, their more intense reduction for immature rats.
Keywords: tobacco smoke, monosodium glutamate, energy supply, rats