O. G. Nesterenko, S. V. Litvinov, N. M. Rashydov
Institute of Cell Biology and Genetic Engineering NAS of Ukraine
ANALYSIS OF THE LTR-RETROTRANSPOSONS MOBILITY IN PLANTS AFTER ABIOTIC STRESS FACTORS IMPACT
The aim of this work was to investigate a comparative analysis of DNA changes in response to different origin stresses and their combinations (osmotic stress, ionizing radiation, heating) in the genome of Pisum sutivum. It can be done using repetitive mobile elements LTR-retotransposons and detecting the appearance of new amplicons on electrophoregrams. Investigation have shown that various types of acute stressors and their combinations can activate retrotransposons mobility in plants and lead to their proliferation by “switching off” epigenetic silencing. Most reactive ability to stress were retrotransposons after using iPBS2074 and СYCLOP primers. Among the stress factors and their doses the most effective for activating transposition of retrotransposons affected ionizing radiation in high (20-25 Gy) and low (5 Gy) doses combined with salinity or without it as well as heating and osmotic shocks by separately were applied.
Keywords: ionizing irradiation, osmotic shock, hyperthermia, Pisum sativum L., stress, retrotransposon