N. M. Luhinich, I. V. Gerush, I. M. Yaremiy, N. V. Davydova
Higher State Educational Establishment of Ukraine "Bukovinian State Medical University" (BSMU)
THE INFLUENCE OF 14 DAYS INTRODUCTION OF MELATONIN ON THE METABOLISM OF GLUTATION IN THE LIVER OF ALLOXAN DIABETIC RATS
Diabetes mellitus (DM) is one of the most prevalent endocrine disorders in the world. Glutathione is a cofactor of many enzymes involved in the detoxification of active forms of oxygen and its deficiency leads to the progression of diabetes. Published data show that melatonin may play a role in glucose metabolism. Introduction of melatonin inhibits peroxidation of lipids and changes in the activity of antioxidant enzymes. Therefore, the purpose of study was to evaluate effect of melatonin on the state of the glutathione system in the liver of rats under conditions of aloxane diabetes mellitus. Experiments were conducted on white outbred sexually mature male rats. The glucose level was measured in the blood. In the liver, the content of reduced glutathione (GSH), activity of glutathione peroxidase (GPx), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G-6-PD) and glutathione-S-transferase (GST). The results of our studies showed that in the liver of rats with diabetes, there was a decrease in the content of GSH and the activity of glutathione-dependent enzymes GPX, G-6-PD and GST compared to control rats. The introduction of melatonin intragastrically over a period of 14 days had a normalizing effect on blood glucose level, GSH content and activity of GPX in the liver of rats in experimentally alloxane-induced DM. Melatonin in the dose of 10 mg/kg contributes to the normalization of blood glucose level and some indicators of glutathione antioxidant defense system in the liver of rats in experimentally alloxane-induced DM, which testifies that its effective antioxidant role in this organ.
Keywords: diabetes mellitus, melatonin, alloxan, glutathione, antioxidant system