В. А. Хоменчук, В. Я. Быяк, Б. З. Ляврин, В. З. Курант

Тернопольский национальный педагогический университет имени Владимира Гнатюка

КОРРЕЛЯЦИОННЫЙ АНАЛИЗ СОДЕРЖАНИЯ МЕТАЛЛОВ И ПОКАЗАТЕЛЕЙ БЕЛКОВО-НУКЛЕИНОВОГО ОБМЕНА В ОРГАНИЗМЕ ПРЕСНОВОДНЫХ РЫБ

Проанализированы корреляционные связи (коэффициент корреляции Пирсона) между количеством аккумулированных металлов в тканях рыб (карп – Cyprinus carpio L., карась – Carassius carassius L., окунь – Perca fluviatilis L., щука – Esox lucius L.) и отдельными метаболическими показателями в их тканях (содержание рибонуклеиновой кислоты (РНК), активности аминотрансфераз (АЛаТ, АСаТ) и сукцинатдегидрогеназы (СДГ). Показано, что показатели корреляции между содержанием металла и отдельными метаболическими показателями в пресноводных рыб зависят от природы металла и являются видо - и тканеспецифическими. Полученные показатели могут быть использованы для оценки загрязнения водных экосистем металлами.

Ключевые слова: рыбы, металлы, рибонуклеиновая кислота, аминотрансфераза, сукцинатдегидрогеназа, корреляция

V. O. Khomenchuk, V. Ya. Biyak, B. Z. Lyavrin, V. Z. Kurant

Ternopil Volodimir Hnatiuk National Pedagogical University, Ukraine

CORRELATIONAL ANALYSIS OF CONTENTS OF METALS AND INDICATORS OF PROTEIN-NUCLEIN METABOLISM IN ORGANISM OF FRESHWATER FISH

Correlational relations (Pearson correlation coefficient) between the amount of accumulated metals in fish tissues (carp – Cyprinus carpio L., crucian carp – Carassius carassius L., perch – Perca fluviatilis L., pike – Esox lucius L.) and the individual metabolic parameters in their tissues (the content of ribonucleic acid (RNA), the activity of the alanine aminotransferase (AlT), aspartate aminotransferase (AspAT) and succinate dehydrogenase (SDH). It has been established that there is a clear correlation between the content of metals and RNA in fish tissues. A positive correlation between accumulated amount of cobalt, manganese and zinc and the level of nucleic acids in the muscles of all fish species under research was shown. There is a positive correlation between the content of cobalt, manganese and zinc and the amount of RNA in the liver of all investigated fish species. Negative correlations of all species are indicated between the amount of manganese and the content of RNA in a liver. There are complex correlational relations between the activity of AspAT and the content of metal ions in the liver of investigated species of fish. It should be noted that the activity of the enzyme decreases zinc content in the liver of crucian carp and pike, increasing activity of AspAT with an increased accumulation of iron, cobalt and copper in the liver of the pike. It should be noted that the more sensitive is the correlation between the content of metals and the activity of the AlT in the liver (especially in the perch), where it is clearly observed reduction of enzymatic activity against the background of the growth of the amount of metals. It is indicated that for liver, in comparison with gills of investigated species fish, the probable correlation relations are more likely to be traced between the accumulation of metals and the activity of succinate dehydrogenase. This may be due to the fact that the liver is an organ that stores significant amounts of metals. A correlations coefficient between the amount of metals and individual metabolic parameters in tissues of freshwater fish can be used to evaluate the pollution by metals in water ecosystems.

Keywords: fish, metals, ribonucleic acid, aminotransferase, succinate dehydrogenase, correlation