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ЗМІНИ ЗАПАЛЬНИХ ТА БІОЕНЕРГЕТИЧНИХ ПРОЦЕСІВ У ЩУРІВ, УРАЖЕНИХ НАТРІЮ НІТРИТОМ ТА ТЮТЮНОВИМ ДИМОМ, ПІСЛЯ ЗАСТОСУВАННЯ МІЛДРОНАТУ

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П .Г. Лихацкий

ГВУЗ «Тернопольский государственный медицинский университет имени И. Я.Горбачевского МОЗ Украины»

ИЗМЕНЕНИЯ ВОСПАЛИТЕЛЬНЫХ И БИОЭНЕРГЕТИЧЕСКИХ ПРОЦЕССОВ У КРЫС, ПОРАЖЕННЫХ НАТРИЯ НИТРИТОМ И ТАБАЧНЫМ ДЫМОМ, ПОСЛЕ ПРИМЕНЕНИЯ МИЛДРОНАТА

Установлено, что поражение крыс разных возрастных групп в течение 30 дней табачным дымом приводит к нарушению дисбаланса в системе про- и противовоспалительных цитокинов, который еще больше углубляется при отравлении токсикованных крыс натрия нитритом. Наиболее выраженные изменения в активности воспалительных процессов отмеченные через 72 ч после поступления натрия нитрита в организм отравленных табачным дымом неполовозрелых крыс. Одновременно с развитием воспалительных процессов происходят нарушения в системе энергообеспечения, что проявляется подавлением активностей митохондриальных ферментов в печени и легких крыс всех возрастных групп после интоксикации. Отмечается угнетение активностей сукцинатдегидрогеназы и цитохромоксидазы, которые наиболее выражены у неполовозрелых крыс. Применение милдроната привело к нормализации выявленных нарушений в функционировании биоэнергетической цепи и уменьшило проявления воспалительных процессов в организме, что подтверждает его антигипоксантные свойства и позволяет рекомендовать для использования при патологиях, сопровождающихся возникновением гипоксических состояний.

Ключевые слова: табачный дым, натрия нитрит, милдронат, воспалительные процессы, процессы энергообеспечения, цитокины

 

P. H. Lykhatskyi

I. Horbachevsky Ternopil State Medical University, Ukraine

CHANGES OF INFLAMMATORY AND BIOENERGETIC PROCESSES IN RATS AFFECTED BY NITRIC AND TOBACCO SMOKE, AFTER APPLICATION OF THE MILDROONATE

Today in Ukraine there are almost 9 million active smokers, which make up one third of the total working population of the country. The versatility of tobacco intoxication is confirmed by the fact that it is associated with the development of almost 40 different diseases in humans. In addition, the pollution of the environment with industrial waste, mineral fertilizers, such as nitrates and nitrites, leads to their negative impact on health on population .. It is relevant to study the mechanisms of the combined action of toxicants on the body and the search for adequate methods for correction of the violations detected. The aim of the work was to study the activity of inflammatory and bioenergetic processes in the body of rats of different ages infected with sodium nitrite against the background of tobacco intoxication, after application of mildronate. Experiments were carried out on white rats, sexually immature, sexually mature and aged, who were exposed to tobacco smoke during 30 days. Experimental animals are divided into 4 groups. One of them, 24 hours before the end of the experiment, was given sodium nitrite at a dose of 45 mg / kg body weight, the second - sodium nitrite was injected 72 hours before euthanasia. In two other groups, after the defeat, both toxicants intrigastically injected the mildronate preparation at a dose of 120 mg / kg body weight for 15 days (from 15 days of intoxication with tobacco smoke and daily until the end of the experiment). In the course of research, the general principles of experiments on animals, in accordance with the provisions of the European Convention for the Protection of Vertebrate Animals, were used. It has been established that the lesion of rats of different age groups during 30 days of tobacco smoke leads to an imbalance in the system of pro-anti-inflammatory cytokines, which further deepens when poisoning toxic rats sodium nitrite. The most pronounced changes in the activity of inflammatory processes are marked 72 hours after the ingestion of sodium nitrite in the body of tobacco-smoke of immature rats. At this time, the content of proinflammatory IL-6 interleukin increases most and the content of anti-inflammatory IL-4 interleukin decreases. Activation of inflammatory processes in the body of rats after sodium defeat with nitrite and tobacco smoke caused changes in the processes of energy supply of cells, manifested by inhibition of activity of mitochondrial enzymes in the liver and lungs of rats of all age groups after intoxication. The most susceptible to the effect of tobacco smoke were the lungs of the sexually immature rats, the activity of SDH in them decreased by 1.4 times. Sodium poisoning with nitrite as an additional toxicant affected by smoke of rats caused even more decrease in the activity of this enzyme in the lungs of rats of all age groups (the lowest was found in immature rats). In the liver of rats, after 30 days of intoxication with tobacco smoke, the activity of CO decreased in immature immature ones by 1.5 times, in the mature and aged in 1.1 and 1.2 times, respectively. The use of sodium nitrite has deepened poisoning of toxic rats and led to the development of hypoxia, as evidenced by the decrease in the activity of CO in the liver of immature animals in 2.2 times, in two other age groups - 1.3 times compared with the level of intact control. The use of mildronate has led to the normalization of the revealed disorders in the functioning of the bioenergy chain and reduced the manifestation of inflammatory processes in the body, which confirms its antihypoxant properties and allows it to be recommended for use in pathologies that are accompanied by the occurrence of hypoxic conditions.

Keywords: tobacco smoke, sodium nitrite, mildronate, inflammatory processes, energy supply processes, cytokines

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