Е. С. Коробкова, Т. В. Затовская
Институт микробиологии и вирусологии имени Д. К.Заболотного НАН Украины
ВЛИЯНИЕ РИЗОБИЙ НА ПРОЯВЛЕНИЕ МИКОПЛАЗМЕННОЙ ИНФЕКЦИИ ЛЮЦЕРНЫ В ЛАБОРАТОРНЫХ УСЛОВИЯХ
В условиях микровегетации исследовано влияние азотфиксирующих клубеньковых бактерий на растения люцерны, инфицированные молликутами, а именно, проведено морфологическое сравнение образцов M. sativa при различных комбинациях инфицирования ахолеплазмами и ризобиями. Установлено, что образование симбиотических связей растений люцерны с R.meliloti 425а способствует улучшению состояния растений и ослаблению негативного влияния на них со стороны фитопатогенных молликутов.
Ключевые слова: молікути, фітопатогенні ахолеплазми, ризобії, симбіоз
K. S. Korobkova, T. V. Zatovska
D. K. Zabolotny Institute of Microbiology and Virology, Ukraine/h4>
EFFECT OF RHIZOBIA ON THE MANIFESTATION OF MYCOPLASMA INFECTION IN ALFALFA UNDER LABORATORY CONDITIONS
Since effective means to fight phytopathogenic mollicutes are almost non-existent, there is a growing need to identify new ways to minimize the harmful effects of these microorganisms on crops. Therefore, the present study aimed to examine the interaction of extracellular vesicles of mycoplasma Acholeplasma laidlawii PG-8 and A.laidlawii var.granulum 118 with alfalfa plants (Medicago sativa) inoculated by symbiotic microorganisms of Rhizobium meliloti 425a. The influence of nitrogen-fixing bacteria on alfalfa plants infected by mollicutes has been studied under conditions of micro-vegetation. In particular, the morphological comparison of M. sativa plant samples infected by acholeplasmas and rhizobia in various combinations was carried out. It should be noted that in the early stages of infection by Acholeplasma laidlawii cells the plants not inoculated by rhizobia displayed growth. Thus, the plants of alfalfa showed such effects of mycoplasmosis as yellowing, growing new leaves and their reshaping. The study demonstrated that the symbiotic relationships between alfalfa and R.meliloti 425a contributes to the improvement of plant state and reduces the negative effects of plant pathogenic mollicutes. In addition, the research proved that the introduction of microbial cultures in the early stages does not affect the length and branching of roots.
Keywords: mollicutes, plant pathogenic acholeplasma, rhizobia, symbiosis