H.I. FALFUSHYNSKA, L.L GNATYSHYNA, O.О. FEDORUK, O.B. STOLIAR

Volodymyr Hnatiuk Ternopil National Pedagogical University, Ukraine, I.Ya. Horbachevsky Ternopil State Medical University

Frogs are susceptible to absorption of toxicants in water through all life stages. The aim of this study was to evaluate the effect of waterborn engeneering nanocomposites (NCs) – Co-NC and Zn-NC, correspondent concentrations of Co2+ (50 µg/L), Zn2+ (100 µg/L), or utilised polymeric substance (PS) on male frog Rana ridibunda during 14 days. Levels of vitellogenin and nuclear lesions (NL) in the blood, cytochrome P450-dependent monooxygenase (CYP450) and glutathione S-transferase (GST) as well as the distribution of Zn, Co, Cu, Cd in the liver were determined. Zn, Zn-NC and PS caused toxic manifestations: oesrogenic effect (Zn and PS), GST downregulation (Zn-NC), NL elevation (Zn-NC and PS). Co-NC only upregulated CYP450, while all other exposures inhibited it. The ratio (Cu+Co)/(Zn+Cd) in metallothionein was increased by the exposures to Co and Co-NC, whyle Zn, Zn-NC and PS increased it in other compounds. This remarkable selectivity distinguished frog from studied previously Carassius sp.

Keywords: cobalt, zinc, nanoscale composite, Rana ridibunda, vitellogenin, metallothionein, cytotoxicity